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Return to UV/Visible
Spectrophotometers
Optional Applications
If your work is focused on Life Science and demands the next level of complexity from our analysis, the DU 800 UV/Visible Spectrophotometer is the right choice for you. Simply select the appropriate applications and add them to the basic system.
Wavelength Scan II
(Extends the capabilities of Wavelength Scan)
Wavelength Scan II adds capabilities to the basic Wavelength Scan application to
provide a more comprehensive and full-featured analytical program.
Extended Key Features
- 1st through 4th derivatives, using the Savitzky-Golay method.
- Log10 for the y-axis.
- The Scatter Correction feature allows a spectrum of the scatter component to be subtracted from the sample spectrum.
- Net A can be determined in a spectrum (net A2, net A3) using a baseline correction.
- Point Picker tabulates data of up to 12 user selected wavelengths.
- Up to 30 peaks or valleys can be detected with the Peak/Valley Picker.
Nucleic Acid Analysis II
(Extends the capabilities of Nucleic Acid Analysis)
The DNA/RNA/Oligo Quant mode adds capabilities that simplifies the acquisition and processing of data from oligo DNA/RNA samples. It determines molecular weight, absorptivity (extinction coefficient), concentration, and theoretical melting temperature for oligo DNA samples. It also simplifies the quantitation of single- and double-stranded DNA and RNA to include corrections for sample dilution and cell
pathlength.
Extended Key Features
- Quantitation of long oligo DNA and RNA samples (› 30 mers) from base composition.
- Quantitation of short oligo DNA and RNA samples (<= 30 mers) from sequence.
- The DNA oligo modes allow you to determine the theoretical thermal melting point (Tm) for DNA oligonucleotide samples.
- Pre-configured modes let you quantitate single- and double-stranded DNA and RNA samples quickly and easily.
- The double ratio method (e.g., 260/280 and 260/230) provides an even better way to determine the purity of your samples; with and without nucleic acid quantitation.
- A scan can be displayed for each sample with markers that verify the accuracy of the selected wavelengths.
Protein Assay Analysis
This program has been optimized for calculating protein concentration with the most commonly used assay methods, such as:
- Bradford
- Lowry (High and Low Sensitivity)
- Biuret
- Direct UV at 280 nm
- Colloidal Gold
- Bicinchoninate (BCA)
- User-Defined Wavelength
Statistical analysis is performed on the standard curve data. The operator can then rerun any standard, omit or include standards on screen, and have the instrument automatically recalculate the curve. Standards and/or coefficients may be stored separately to be re-used.
Key Features
- Linear regression fit with zero or non-zero intercept.
- Non-linear (quadratic) regression.
- Use of up to 30 standards (in duplicate or triplicate, if desired).
- Optimal wavelength for assay is pre-selected.
- Individual standard selection with ability to omit or rerun standards that are out-of-range.
- Review and/or edit standard curves prior to storage and/or processing of samples.
- Zoom, trace, curve offset, autoscaling and annotation.
- Statistical analysis of sample data; variance and r2.
- Use of up to three replicates for individual samples.
- Dilution correction for each sample.
Enzyme Mechanism
Allows for for rapid and easy characterization of a wide variety of enzyme reactions. The program calculates
Km, Vmax, kcat and Ki as well as the Hill constant. It will also display data plots, such as Michaelis-Menten, Lineweaver-Burk, Eadie-Hofstee, Hanes-Woolf, and the Hill and Inhibitor plots. Competitive, non-competitive or uncompetitive enzyme reaction mechanisms can be easily determined and/or confirmed.
Key Features
- Automatic blank subtraction and background correction for up to 24 blanks and samples.
- Abs/min for each sample and time point.
- Reaction rates can be determined automatically by the instrument or from user selected time points.
- Choice of rate calculation methods: linear, auto-linear, first order, and auto-first order.
- Km, Vmax, kcat, and Ki
determinations by Marquardt nonlinear regression analysis.
- Plots generated include:
- Michaelis-Menten
- Lineweaver-Burk
- Eadie-Hofstee
- Hanes-Woolf
- Inhibition
- Hill
- Hill constant calculation.
- Calculation of Ki for competitive, uncompetitive, and noncompetitive mechanisms.
- Zoom, trace, curve, offset, autoscaling and annotation.
Enzyme Activity
There are many instances where enzyme activity measurements, typically with large number of samples, are required. These include assays of chromatography column fractions in the purification of an enzyme, quantitation of fusion protein products, and promoter analysis with gene fusion to an enzyme.
Key Features
- One or two time points may be chosen for the assay.
- User may input activity factor and desired activity "units".
- Replicate assays, up to three.
- Sipper and Batch Sampler recommended for large number of samples.
Experimental Tm Analysis
(Available as an Accessory Only - See below)
All components of the Micro Tm Analysis Accessory were designed to exclusively perform Experimental Tm Analysis. All components are optimized for simplicity and convenience, small sample volumes, effective heat transfer, accurate temperate control and high sample throughput. Denaturation and renaturation studies of DNA samples can be performed with up to six 325 µL samples at one or two wavelengths. The system provides three calculation methods, first derivative, two-point average and non-linear fit.
Key Features
- Peltier temperature-controlled Micro Tm Cell Holder.
- Integrated temperature sensors; no external temperature probe required.
- Efficient heat transfer and accurate temperature control is achieved by the use of two high-performance peltier elements, low mass cells and optimally positioned temperature sensor.
- Excess heat is quickly removed by the fan in the transport; no water cooling required.
- Purge connector to purge cells with nitrogen; required when working below room temperature to prevent condensation on the surface of the cells.
- Specially designed stoppered 1 cm path length microcells for sample volumes of only 325 µL.
- High performance temperature controller with a temperature range from 0 to 110°C.
- Select one or two analytical wavelengths, or one analytical and one background wavelength.
- Ramp rate selection from 0.1 to 5.0°C/min.
- Up to three temperature ramps per analysis, including read interval for each ramp and delay time.
- Each position in the cell holder can be assigned either to a sample, blank or marked as empty.
- Reverse experiment and dwell time selection.
- Three calculation methods: first derivative, two-point average and non-linear fit.
The application requires the High Performance Transport (P/N 517040) and the Experimental Tm Analysis Accessory (P/N 390263 for 120V or 390264 for 240V), which includes:
- High Performance Temperature Controller (120V or 240V)
- 325 µL Tm Stoppered Microcells (package of 6 cells)
- Micro Auto-6 Tm Cell Holder
- Tm Analysis Software Option
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