Performance Testing

Column Conditions

• Column Operating Conditions
  • pH Range: pH 2.5 to pH 7.5
  • Temperature Range: 0 to 45° C
  • Solvent Filtration: Use 0.5 mm filters standard or large-pore filter for viscous solvents
  • Maximum backpressure - 5000 psi
• Solvent Compatibility
  • Useful Solvents: Hexane, Methylene Chloride, Isopropanol, Tetrahydrofuran
  • Storage Solvents: Hexane, Heptane
  • Solvents to Avoid: Water, Methanol, Chloroform, Solvents pH < 2.5 and > 7.5
• Regeneration Procedure
  • Flush with 20-column volumes each, in the following sequence. Start with solvent next highest in polarity to the solvent presently in the column. Equilibrate after each step. Hexane -> Methylene Chloride -> Isopropanol -> Methylene Chloride -> Hexane

Ultrasphere CN, 80 Å Pore

• Better reproducibility. Less susceptible to the effects of small solvent changes (such as the water content in non-polar solvents)
• Highly versatile. Can be used in either normal or reversed phase HPLC
• Based on the same 5 µm silica as the Ultrasphere ODS line

Polar-bonded phase columns serve a dual purpose. They may be used in normal phase as well as reversed phase modes of liquid chromatography. Separations on these columns utilize solvents that are of lower polarity with respect to the stationary phases that are especially desirable for separating compounds belonging to the same homologous series.

Ultrasphere CN is especially designed for application where a moderately polar-bonded phase material is desired.

Ultrasphere Si, 80 Å Pore

• High loading capacity and strong mechanical stability due to exceptionally stable silica packing.
• Minimal peak distortion

Adsorption columns containing silica are among the most polar of all packing material-useful for separating compounds differing in the number and type of the functional group.

The basis of retention in adsorption chromatography with silica is a specific interaction between sample molecules and the silica surface. This technique generally applies to solutes which are soluble in nonpolar solvents are not ionized. It is widely used for the separation of stereoisomers as well as neutral and weakly acidic compounds.

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