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Important: You must have either the CEQ 2000XL version 4.3 or the CEQ 8000 V5.0 installed before installing the new CEQ Quality Values tables. Introduction: The CEQ Genetic Analysis Systems produce two estimated error probabilities for each called base. The estimated error probabilities aid users in performing various data analysis tasks including: quality weighted sequence assembly, sequence trimming for various purposes including unweighted sequence assembly, primer selection, single-pass (e.g. EST) sequence editing, quality-weighted homology searching, etc. The first estimated error probability is called the Quality Value (QV) and provides an estimate that will discern between various degrees of high-quality ("low" error probability) bases for purposes such as quality-weighted sequence assembly and automated primer selection. The second estimated error probability is called the Call Score (CS) and provides an estimate that will discern between various degrees of lower-quality ("high" error probability) bases for purposes such as sequence trimming and single-pass sequence editing. Both QVs and CSs can be viewed in the Sequence Analysis software module of the CEQ. QVs are exported as part of the PHD file export and as part of the FASTA file export. CSs can only be exported as part of the Text file export. The download file below contains the updated versions of the Tables that were rebuilt using over 9 million bases. Instructions:
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.
Additional GenomeLab™ Information:
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