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The History of Beckman Coulter in Capillary ElectrophoresisBeckman Coulter (then Beckman Instruments) introduced the first fully automated capillary electrophoresis system -- the P/ACE™ 2000 -- in 1989. Since that time, Beckman has continued to develop the technology, introducing numerous product enhancements and application developments, all of which have contributed to the growing acceptance and application of CE. Beckman Coulter CE Milestones:1989: Introduction of the P/ACE 2000 1990: P/ACE 2000 detection enhancements and a new rebuildable capillary cartridge design 1991: Introduction of the first LC-CE system, linking liquid chromatography and CE under the System Gold™ control and analysis workstation. In the same year, Beckman introduced the first in its line of eCAP™ chemistry kits – the ssDNA 100 chemistry for oligonucleotide purity analysis. 1992: Beckman introduced the world's first laser-induced fluorescence (LIF) detector for CE, increasing sensitivity up to 1,000 times over UV detection for fluorescing compounds. Chemistry kits for the size based separation of proteins, as well as a CE-MS adapter, were also introduced. That same year, Beckman developed significant advancements in sample cooling technology for CE. 1993: Introduction of the P/ACE 2200 System, featuring many enhancements including simultaneous voltage and pressure for the purpose of mobilizing pH gradients. This was followed by the introduction of the P/ACE 5500 system, which featured photo-diode array detection with real time peak purity analysis. Chemistry kits for high sensitivity double-stranded DNA analysis (replaceable gels) were also introduced. 1994: Beckman offers three more chemistry kits – the eCAP Amine Capillary Methods Development Kit for small molecules, the eCAP Amine Methods Development Kit for proteins and the eCAP Neutral Capillary Methods Development Kit. 1995: Introduction of a 635 nm diode laser, as well as the first in a series of chemistry kits for chiral analysis. More new kits were introduced, including the eCAP dsDNA 20,000 Kit for the separation of large DNA fragments, and the eCAP IEF 3-10 kit for isoelectric point determination. Introduction of the first generation Paragon CZE® 2000 system, a seven-capillary array for clinical serum protein analysis. 1996: The introduction of both P/ACE Station software as well as LIF only application systems. The eCAP ssDNA 100-R kit and the eCAP N-linked oligosaccharide profiling kit were also introduced this year. 1997: Beckman introduced the P/ACE MDQ platform, specially designed for research, methods development and quality control in the biotech/pharmaceutical industry. 1998: New highly sulfated cyclodextrins were introduced, along with an external detector adapter and a large-volume buffer tray. Beckman Coulter also developed the first in a series of CD-ROM tutorials on the application of CE technology. Beckman Coulter also launched the CEQ™ 2000 CE-based DNA sequencing system featuring replaceable linear polyacrylamide gels, a multi-capillary (8) separation array, and infrared dyes for sequencing and genotyping. 1999: Beckman Coulter introduced a dual-wavelength LIF detection module for the P/ACE MDQ, along with new application-specific systems for glycoprotein, carbohydrate and DNA analysis. Specialized Fragment analysis software was introduced for the CEQ 2000. 2000: Beckman Coulter, in collaboration with ThermoQuest*, introduces an integrated CE-MS/MS platform. Beckman Coulter also introduces a new 32-bit software platform for CE -- 32 Karat™ Software Version 4.0 -- providing numerous control and analysis functions as well as technical controls for 21 CFR Part 11 compliance. This year also marked the introduction of the CEQ 2000XL DNA Analysis System, delivering faster cycle times with longer reads. 2001: Version 5.0 32 Karat software – adding mobility plots, and mobility/spectrum ID for drug screening applications as well as additional technical controls to assist with 21 CFR Part 11 compliance. The addition of heterozygote detection, mutation detection software and AFLP® applications to the CEQ 2000XL platform. 2002: Introduction of the CEQ 8000 Genetic Analysis System, featuring one gel, one array and one software, as well as a new primer extension based SNP detection chemistry. 2003: Introduction of the ProteomeLab™ PA 800 Protein Characterization System, including the ProteomeLab SDS-MW Analysis Chemistry, Isoelectric Focusing Chemistry and Carbohydrate Labeling and Analysis Chemistry. In the fall 2003 Beckman Coulter also introduced the ProteomeLab IgG Purity/Heterogeneity assay for use on the PA 800 Platform and the GenomeLab™ CEQ 8800 Genetic Analysis System, which features bar code plate recognition, sample tracking, methods link to automated sample preparation and double the operational capacity of the previous system. Beckman Coulter, Inc.Beckman was founded in 1935 by Dr. Arnold O. Beckman with the invention of the acidometer (modern day pH meter), which is probably the most widely used analytical instrument in the world. Today Beckman Coulter is a leading Biomedical Testing Company employing more than 10,000 people around the world, providing service and support in more than 120 countries. Beckman Coulter, Inc. is a leading manufacturer of biomedical testing instrument systems, tests and supplies that simplify and automate laboratory processes. Spanning the biomedical testing continuum – from systems biology and clinical research to laboratory diagnostics and point-of-care testing – Beckman Coulter's 200,000 installed systems provide essential biomedical information to enhance health care around the world. The company, based in Fullerton, Calif., reported 2003 annual sales of $2.2 billion with 64 percent of this amount generated by recurring revenue from supplies, test kits and services. Common Acronyms:
® AFLP is a registered trademark of Keygene Corporation. * All trademarks are the property of their respective owners. For Research Use Only; not for use in diagnostic procedures.
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