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Elevate Your Research Into a Class of Its Own
CD4+ T cells play a central role in the regulation of both appropriate and inappropriate immune responses. During the course of a normal immune response to foreign invaders such as viruses, bacteria and tumor cells, CD4+ T cells are activated in response to specific antigenic peptides presented to them in the context of MHC Class II molecules. CD4+ T cells mediate a host of downstream immune effector functions including antibody production, activation of cytotoxic T cells and production of other inflammatory mediators. In autoimmune diseases, CD4+ T cells are inappropriately activated by autoantigens resulting in antibody production, and stimulation of CTL activity as well as other inflammatory responses. This inappropriate activation of the immune cascade results in tissue destruction and the pathogenesis of autoimmune diseases including diabetes, multiple sclerosis, lupus and rheumatoid arthritis, and also plays a pivotal role in cancer and infectious diseases.
With Class II iTAg Tetramers to complement existing Class I Tetramers, it is possible to detect and enumerate both CD8+ and CD4+ antigen specific T cells - allowing you to see the whole picture of immune response. Measurement of CD4+ T cells with Class II Tetramers could be used to evaluate antigen-specific immune function in the evaluation of vaccine therapy, immunotherapy, immune competence, disease risk analysis, disease prognosis and cellular anergy.
- Detect and quantitate CD4+ antigen-specific T cells
- Class II Tetramer applications include Type I diabetes, rheumatoid arthritis, multiple sclerosis, lupus, HIV, cancer and other diseases
- Available alleles: DRB1*0401, DRB1*0101, DRB1*1501, DRB1*0301, DPB1*0401
Detection of Influenza Hemagglutinin (HA) Specific CD4+ T Cell Clone (B16) Using DR*0401 HA iTAg Tetramers
A
A) Whole blood (unspiked) |
B
B) Whole blood spiked with 1%
B16 cells |
C
C) Whole blood spiked with 5%
B16 cells |
Linearity: Whole Blood Spiked with 0-50% B16 Cells Stained with DR*0401 HA iTAg Tetramers
Human whole blood spiked with DR4 positive T cell clone B16 at 0-50%. Figure shows observed mean % recovery of spiked positive T cell clone B16 into normal human whole blood compared to expected values.
Negative Tetramer
The Negative Tetramer is used to assess non-specific binding in iTAg MHC Tetramer Analysis. Negative Tetramer is used with all Class I human alleles.
Panel A: CD3-FITC /iTAg Negative Tetramer SA-PE
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Panel B: CD8-FITC/iTAg A*0201 CMV pp65 Tetramer SA-PE
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