
Image analyses are and have always been indispensable
tools in particle studies. Conventional microscopic analysis has advantages
over other methods in that it can provide information on size, shape,
surface texture and some optical properties of individual particles
in great detail. Microscopic analysis has often been the final judge
of the size of monodisperse standard reference materials. However,
the biggest drawback of conventional microscopic analysis is that
the number of particles in focus that can be inspected in any field
of view is limited. Thus, for a polydisperse sample, an adequate statistical
representation of the entire sample can be an exhaustive, if not impossible,
task.
Conventionally, captured images from microscopic measurements
are recorded on photographic papers followed by manual study of the
shape, size or surface morphology of the particles in the images.
Nowadays, using computer automation, images can be captured and viewed
directly on a monitor and digitized and recorded into computer files
at a rate of millisecond or even microsecond. These images then can
be reprocessed (e.g., by image enhancement) and analyzed using an
image analysis program. Particle dimensional measurement (size, area,
or cord length), particle count, shape analysis, and even fractal
analysis can be accomplished by image analysis. Because of its rapid
image capturing capability, more particles can be analyzed by continuously
delivering them, either in liquid or in air, to the location of image
capturing. This technique is then called dynamic image analysis.
In
a dynamic image analysis device, the image capture procedure is accomplished
using illumination of light combined with a sample cell and a CCD
device. A frame grabber card then transfers the image to computer
for further analysis. Depending on the optic setup, the size range
of particles that can be analyzed using a dynamic image analyzer is
between a few microns to a few millimeters.